Abcam, ab76198, Anti-eNOS antibody [M221]

Size: 100µL
Anti-eNOS antibody [M221] (ab76198) is a mouse monoclonal antibody detecting eNOS in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse . - Over 170 publications - Trusted since 2009
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:M221,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Mouse Nos3 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P70313,
Specificity:ab76198 is not predicted to react with other NOS family members due to low homology.This antibody detects eNOS in mouse and but at a lower intensity than in human. If you are working in mouse, we would recommend using no more than 1% milk as the blocking agent for optimal signal strength.

Product details:
What is this antibody validated in?
Anti-eNOS antibody [M221] (ab76198) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of eNOS?
Anti-eNOS [M221] (ab76198) specifically detects a band for eNOS (UniProt: P70313) at a molecular weight of 133kDa.
Trusted by the scientific community
Anti-eNOS [M221] (ab76198) was first used in a scientific publication in 2009 and has been cited over 170 times in peer-reviewed journals.
Reviewed by scientists
Anti-eNOS [M221] (ab76198) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
Biological function summary
ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.
Pathways
ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.
ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.