Abcam, ab76572, Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal JNK1 phospho Y185 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 91 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1597Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Dot, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The JNK protein family also known as c-Jun N-terminal kinases consists of JNK1 JNK2 and JNK3. These proteins are mitogen-activated protein kinases (MAPKs) that play a role in transmitting extracellular signals to the cell nucleus. JNK1 has a mass of approximately 54 kDa and is ubiquitously expressed in various tissues. JNK2 is similar in mass and expression while JNK3 is primarily found in the brain heart and testes. These proteins can phosphorylate transcription factors such as c-Jun impacting gene expression processes.
Biological function summary
The JNK proteins are involved in controlling cellular events like proliferation differentiation and apoptosis. They act as part of the MAPK signaling complex and involve scaffolding proteins that help organize these signaling pathways. In the cellular context JNK proteins are activated in response to stress signals like UV irradiation inflammatory cytokines and growth factors. This activation leads to diverse cellular outcomes depending on the specific JNK isoform and cell type.
Pathways
The JNK proteins are important in the MAPK signaling pathways and play a role in the AP-1 transcription factor regulation. They interact with proteins such as MEK4 and MEK7 which are upstream kinases that activate JNK through phosphorylation. The JNK signaling arm is distinct from the ERK and p38 MAPK pathways each having specific roles in cellular response modulation.
The JNK proteins are implicated in conditions like neurodegenerative diseases and cancer. In Alzheimer's disease for example JNK3 contributes to neuronal cell death while JNK inhibitors like SP600125 have been explored for therapeutic potential. In cancer JNK proteins can influence tumor progression through the regulation of apoptosis and their activity may intersect with other kinases like p53 further complicating disease pathways. JNK's role in diseases makes it a critical target for developing inhibitors reflected in studies around compounds like JNK inhibitor 8 and related molecules.