Abcam, ab77088, Anti-PIWIL3 antibody

Size: 50µg
Mouse Polyclonal PIWIL3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PIWIL3.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PIWIL3.Q7Z3Z3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIWIL3 also known as P-element-induced wimpy testis-like 3 is a member of the Argonaute protein family. It plays an important role in the regulation of gene expression through a mechanism called RNA silencing. PIWIL3 is involved in the formation of piRNA complexes which are important in silencing transposable elements and protecting genomic integrity. This protein has a molecular mass of approximately 97 kDa. PIWIL3 is expressed mainly in the testes but also in other tissues such as placenta and ovaries.
Biological function summary
PIWIL3 functions to maintain genomic stability by forming complexes with piRNAs that guide the protein to transposon targets in a sequence-specific manner. It works alongside other PIWI proteins and associates with components of the piRNA biosynthetic machinery. PIWIL3 helps to repress the activity of transposable elements preventing their harmful insertion into the genome and ensuring proper germline cell development. It forms complexes with TDRD proteins which assist in piRNA biogenesis.
Pathways
PIWIL3 is involved in the piRNA metabolic pathway that is essential for germline maintenance and genome defense. It connects with other PIWI family members like PIWIL1 and PIWIL2 in this pathway coordinating their actions to ensure efficient silencing of transposable elements. The interactions of PIWIL3 with proteins in the argonaute and RNA-induced silencing complex (RISC) pathways highlight its role in post-transcriptional gene regulation which is important for proper cell differentiation and development.
PIWIL3 expression levels and functionality have been associated with certain cancers particularly testicular cancer and ovarian cancer. Aberrant PIWIL3 activity due to dysregulation in transposable element silencing may contribute to oncogenesis by allowing genomic instability. The protein connects with disease pathways where it interacts with other RISC-related proteins such as Dicer and Ago2 which are involved in microRNA processing and gene silencing mechanisms.