Abcam, ab77144, Anti-Spastin antibody [Sp 6C6]

Size: 50µg
Mouse Monoclonal Spastin antibody. Suitable for WB, IP and reacts with Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPAST.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Sp 6C6,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human SPAST.Q9UBP0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Spastin also known as SPG4 is a microtubule-severing ATPase that plays an important role in cellular mechanics by cutting microtubules in cells. It weighs approximately 68 kDa and is present mainly in neural tissue but can also be found in other tissues. The protein's localization includes the cytoplasm and is associated with centrosomes and subcellular regions with dense microtubule networks.
Biological function summary
Spastin participates in regulating microtubule dynamics. It plays a critical role in axonal growth and maintenance by modulating microtubule lengths and branching. Spastin does not function alone; it forms part of the AAA protein family functioning in significant processes like neurogenesis and neural maintenance. Its activity affects neural cell structure and transport by influencing cytoskeletal rearrangement.
Pathways
Any disturbances in Spastin function can impact the microtubule dynamics pathway and the axonal transport pathway. Spastin interacts closely with proteins such as Tubulin and Katanin which are also involved in microtubule severing and organization. Proper Spastin function is necessary to maintain intracellular transport and signal transduction critical for neural cell health and function.
Alterations in Spastin activity lead to neurodegenerative conditions like Hereditary Spastic Paraplegia (HSP). This disorder often relates to mutations in the SPG4 gene which encodes Spastin affecting motor neuron functions. In HSP disrupted Spastin function correlates with deficiencies in proteins such as Tubulin exacerbating neuroaxonal deficits and influencing disease progression.