Abcam, ab77231, Anti-PIAS1 + PIAS2 antibody [EPR2581Y]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PIAS1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Synthetic peptide - Human, Mouse, Rat samples. Cited in 21 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2581Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIAS1 and PIAS2 which stand for Protein Inhibitor of Activated STAT1 and STAT2 respectively are members of the PIAS protein family. These proteins are known to function as E3 SUMO-protein ligases and play a significant role in the post-translational modification process called SUMOylation. PIAS1 has a mass of about 71 kDa and PIAS2 has a mass of approximately 73 kDa. PIAS1 and PIAS2 are expressed in various tissues with high expression observed in the brain and hematopoietic cells.
Biological function summary
PIAS1 and PIAS2 play essential roles in modulating key signaling pathways through SUMOylation. They often work as part of larger protein complexes modulating the activity of transcription factors such as STAT p53 and NF-kB. By affecting these transcription factors PIAS proteins can regulate gene expression thereby influencing cell proliferation differentiation and response to stress. This involvement in gene regulation underlines their importance in maintaining cellular homeostasis.
Pathways
PIAS1 and PIAS2 contribute to critical processes like the JAK-STAT signaling pathway and the NF-kB signaling pathway. These pathways are influential in controlling immune responses. Through phosphorylation and interaction with other transcription factors like STAT1 and NF-kB PIAS proteins alter the transcriptional activity of genes involved in inflammation and cellular stress responses. The modulation of these pathways links PIAS1 and PIAS2 with both immune and stress responses playing a role in both normal and pathological conditions.
PIAS1 and PIAS2 are associated with chronic inflammation and cancer. Their regulatory role in the JAK-STAT and NF-kB pathways connects them to inflammatory diseases where dysregulation of these pathways is common. Additionally PIAS1 and PIAS2 interact with proteins like p53 linking them to tumor suppression and anomalies in their expression or function can contribute to the development of cancers. Understanding these associations is important for developing therapies targeting these signaling proteins.