Abcam, ab7753, Anti-pan Cytokeratin antibody [C-11]

Size: 20µg / 100µg / 1mg
Anti-pan Cytokeratin antibody [C-11] (ab7753) is a mouse monoclonal antibody detecting pan Cytokeratin in Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, mIHC . Suitable for Human, Mouse, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Over 140 publications - Trusted since 2001
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:C-11,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, mIHC, Flow Cyt (Intra), ICC/IF, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Cytokeratin peptides 4,5,6,8,10,13,18.

Product details:
This product has switched from a hybridoma to recombinant production method on 08
March 2021
Product Specifications
Anti-pan Cytokeratin antibody [C-11] (ab7753) is a mouse recombinant monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, mIHC in human, mouse, rat samples.
Anti-pan Cytokeratin antibody [C-11] (ab7753) specifically detects pan Cytokeratin (UniProt ID: Q7Z794; Molecular weight: 62kDa) and is sold in a convenient trial size to enable initial testing (20 µg) and larger sizes for subsequent scaling up experiments (100 µg and 1 mg).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-pan Cytokeratin antibody [C-11] (ab7753) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-pan Cytokeratin antibody [C-11] (ab7753) has been cited over 146 times in peer reviewed journals and is trusted by the scientific community.
Anti-pan Cytokeratin antibody [C-11] (ab7753) has 23 independent reviews from customers.
Related Products
Antibody clone C-11 is also available pre-conjugated to a variety of labels for your convenience - PE, APC, Alexa Fluor® 594, Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 647 (
ab52460
ab106166
ab277234
ab277270
ab279324
ab309978
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from TCS. Purity >95% by SDS-PAGE., Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
Biological function summary
Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.
Pathways
Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).
Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.