Abcam, ab7785, Anti-Tropomyosin antibody [TM-311]

Size: 250µL
Mouse Monoclonal TPM1 antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:TM-311,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tropomyosin also known as anti-TM is a coiled-coil protein with a mass typically around 32 to 37 kDa depending on the isoform. This protein exists in both muscle and non-muscle cells playing essential roles in stabilizing actin filaments. In muscle tissues tropomyosin is part of the thin filament structure. It is found in high concentrations in skeletal cardiac and smooth muscles and is responsible for regulating the interaction between actin and myosin which is important for muscle contraction.
Biological function summary
In muscle contraction and cellular movement tropomyosin acts as a regulatory protein. It associates with the troponin complex to control the binding of myosin heads to actin filaments. By shifting its position on the actin filament tropomyosin enables the muscle contraction cycle to proceed. It is an integral part of the thin filament complex interacting with actin and troponin and is essential for the calcium-dependent regulation of muscle contraction.
Pathways
Tropomyosin plays a significant role in the actin cytoskeleton regulation pathway. It influences the dynamics of actin filament stability and organization which are important for cell motility and structure. The protein also interacts with the calmodulin-dependent protein kinase II (CaMKII) pathway where its interaction influences heart and skeletal muscle functionality. In these pathways tropomyosin regulates the activities of proteins such as myosin and actin ensuring proper cellular mechanical function and signaling.
Alterations in tropomyosin function can lead to several muscle-related conditions. Hypertrophic cardiomyopathy and dilated cardiomyopathy are two disorders associated with mutations in tropomyosin. In these conditions improper regulation of the actin-myosin interaction disrupts normal cardiac muscle function. Tropomyosin-related disorders often involve interactions with proteins such as troponin which is another key player in the regulation of muscle contraction and is important for maintaining a healthy cardiac rhythm.