Abcam, ab7800, Anti-Cytokeratin 14 antibody [LL002]

Size: 100µg
Anti-Cytokeratin 14 antibody [LL002] (ab7800) is a mouse monoclonal antibody detecting Cytokeratin 14 in Western Blot, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Over 250 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:LL002,
Isotype:IgG3,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.

Product details:
Anti-Cytokeratin 14 antibody [LL002] (ab7800) is a House Mouse Monoclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human samples.
Anti-Cytokeratin 14 antibody [LL002] (ab7800) has been cited over 259 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-Cytokeratin 14 antibody [LL002] (ab7800) has high sensitivity and specificity.
The specificity of Anti-Cytokeratin 14 antibody [LL002] (ab7800) has been confirmed by testing in knockout samples.
Anti-Cytokeratin 14 antibody [LL002] (ab7800) has 25 independent reviews from customers.
Anti-Cytokeratin 14 antibody [LL002] (ab7800) specifically detects Cytokeratin 14 (UniProt ID: P02533; Molecular weight: 52kDa) and is sold in 100 ug selling sizes.
Antibody clone LL002 is also available pre-conjugated to a variety of labels for your convenience - FITC, Biotin, Alexa Fluor® 555, Alexa Fluor® 488, Alexa Fluor® 647 (
ab77684
ab271819
, ab3266, ab327,
ab321783
Cytokeratin 14 (CK14) is primarily expressed in the basal layer of stratified squamous epithelium and it plays a crucial role in maintaining cell structure and resisting mechanical stress. High levels of CK14 expression are associated with a distinct pattern of metastasis.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.
Biological function summary
This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.
Pathways
Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.
Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.