Abcam, ab78078, Anti-beta III Tubulin antibody [2G10] - Neuronal Marker

Size: 100µg
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) is a mouse monoclonal antibody detecting beta III Tubulin in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Catshark, Common marmoset, Dogfish, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 320 publications - Trusted since 2009
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2G10,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Dogfish, Common marmoset, Catshark, Mouse, Rat, Human,
Applications:ICC/IF, IP, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) was first used in a scientific publication in 1990 and has been cited over 323 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) has high sensitivity and specificity.
The specificity of Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) has been confirmed by WB testing in beta III Tubulin knockout HAP1 cells.
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) has 40 independent reviews from customers.
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) specifically detects beta III Tubulin (UniProt ID: Q4QRB4; Molecular weight: 51kDa) and is sold in 100 ug selling sizes.
Conjugation-ready, carrier free format available for antibody clone 2G10 -
ab264097
Antibody clone 2G10 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, HRP (
ab195879
ab19588
ab196638
TUBB3, also known as beta-III tubulin, is a critical protein in neuro research, particularly for its role as a marker of neuronal differentiation and axonal growth. It is predominantly expressed in neurons and is essential for proper microtubule function and stability. Researchers use TUBB3 to study neuronal development, neurogenesis and the structural integrity of neurons, making it vital for understanding various neurodevelopmental and neurodegenerative conditions.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.
Biological function summary
Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.
Pathways
Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.
Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.