Abcam, ab78237, Anti-CD20 antibody [EP459Y]

Size: 100µL / 1mL
Anti-CD20 antibody [EP459Y] (ab78237) is a rabbit monoclonal antibody detecting CD20 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 70 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP459Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow Cyt (Intra), WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:For optimal performance in IHC, the primary antibody should be incubated overnight at 4?.

Product details:
What is this antibody validated in?
Anti-CD20 antibody [EP459Y] (ab78237) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of CD20?
Anti-CD20 [EP459Y] (ab78237) specifically detects a band for CD20 (UniProt: P11836) at a molecular weight of 33kDa.
Trusted by the scientific community
Anti-CD20 [EP459Y] (ab78237) was first used in a scientific publication in 2009 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-CD20 [EP459Y] (ab78237) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-CD20 antibody [EP459Y] (ab78237) has been confirmed by Western blot testing in MS4A1 Knockout Raji cell line,
ab273871
Other related products
We have a range of other formats of antibody clone [EP459Y] also available for your convenience: ab78237, Carrier free -
ab166865
, Alexa Fluor® 488 -
ab198941
, Alexa Fluor® 647 -
ab198943
, Carrier free -
ab214282
ab279298
ab279299
ab279300
, Carrier free -
ab279310
, Carrier free -
ab279311
, Carrier free -
ab279312
, ab284424, PE -
ab303031
, APC -
ab303032
, HRP -
ab303033
, Alkaline Phosphatase -
ab308897
, Alexa Fluor® 594 -
ab310623
, Alexa Fluor® 555 -
ab312151
, Alexa Fluor® 568 -
ab312637
, FITC -
ab322915
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.