Abcam, ab79635, Recombinant human Src protein

Size: 10µg
Recombinant human Src protein is a Human Full Length protein, in the 2 to 536 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag N-Terminus,
Applications:WB, FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Activity: 1007 pmol/min/μg. Assay conditons: 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml Poly(Glu:Tyr) substrate and 20 uM ATP. Reaction was done at 30°C for 35 min.,
Accession:P12931,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Preservative: 0.851% ImidazoleConstituents: 50% Glycerol (glycerin, glycerine), 0.403% Sodium chloride, 0.394% Tris HCl, 0.04627% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01006% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Src also known as c-Src is a protein-tyrosine kinase involved in the regulation of many cellular processes. The molecular weight of Src is approximately 60 kDa. Src is ubiquitously expressed in human tissues but shows increased expression in specific tissues like the brain and epithelial cells. This protein has several important roles in cellular signal transduction particularly influencing cell growth differentiation and survival.
Biological function summary
The Src protein interacts with other proteins to modulate cell adhesion motility and angiogenesis forming part of larger protein complexes. Src phosphorylates specific tyrosine residues on its substrates altering their activity interaction or stability. This activity positions Src as an important factor in managing cell communication and structural organization. Src's interaction with focal adhesion complexes emphasizes its functionality in cellular structural integrity and intracellular communication pathways.
Pathways
Src plays a critical role in the integrin and growth factor receptor signaling pathways mediating cross-talk between cell surface receptors and intricate signaling cascades. It closely associates with focal adhesion kinase (FAK) within these pathways influencing cytoskeletal rearrangements and cell movement. Src's function in the epidermal growth factor receptor (EGFR) signaling pathway likewise demonstrates its importance in regulating cellular proliferation and survival mechanisms.
Src has significant implications in oncogenesis particularly in colorectal and breast cancers. Overexpression or abnormal activity of Src associates with tumor progression and metastasis. Within these cancers Src cooperates with various proteins like the EGFR amplifying aberrant cell signaling that contributes to uncontrolled cell growth. Investigating Src's role and regulation could offer insights into novel therapeutic strategies for controlling Src activity in cancer treatment.