Abcam, ab80255, Anti-SMAD1/5/9 antibody

Size: 100µg
Rabbit Polyclonal SMAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab80255 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen., Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMAD1/5/9 proteins also known as SMAD family members 1 5 and 9 play key mechanical roles in the TGF-beta signaling pathway. These proteins have molecular masses ranging between 52-60 kDa. They are widely expressed in various tissues with notable expression in embryonic tissues and the central nervous system. These proteins operate as intracellular signal transducers and transcriptional modulators blending both cytoplasmic and nuclear functions.
Biological function summary
SMAD1/5/9 proteins serve critical functions in the cellular response to signaling molecules like bone morphogenetic proteins (BMPs). They form part of a complex with receptor-regulated SMADs (R-SMADs) and common-mediator SMAD4 which translocates to the nucleus to regulate the transcription of target genes. These proteins have roles in processes like bone and cartilage development cell growth and apoptosis engaging as vital mediators in various cellular contexts.
Pathways
SMAD1/5/9 proteins are integral components of the BMP signaling pathway which governs numerous biological functions such as differentiation and embryogenesis. These proteins work alongside partners such as SMAD4 to mediate the transcription of genes involved in these intricate processes. Additionally they interact with proteins in the SMAD pathway modulated by TGF-beta and engage in crosstalk with other signaling pathways to coordinate cellular outcomes.
SMAD1/5/9 proteins have connections to pulmonary hypertension and skeletal malformations. Aberrant activity or dysregulation of these proteins relates to the progression of these conditions. In pulmonary hypertension the abnormal regulation of the BMP pathway often through interactions with endothelial cells involves SMAD1/5/9. In skeletal disorders such as congenital skeletal malformations these proteins influence bone formation and maintenance often working in conjunction with other proteins such as SMAD4 whose disruptions can exacerbate disease states.