Abcam, ab80350, Recombinant human HDAC9 protein

Size: 10µg
Recombinant human HDAC9 protein is a Human Fragment protein, in the 604 to 1066 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: >1000 U/ug. One U =1pmol/min. Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate, and 0.2 ng/ul HDAC9. Incubation condition: 30 min at 37°C.,
Accession:Q9UKV0,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 50% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC9 also known as Histone Deacetylase 9 is a protein involved in removing acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC9 has a molecular weight of about 127 kDa. It expresses predominantly in the brain muscle tissue and immune cells. This protein belongs to the class IIa family of histone deacetylases and has close structural similarities to other class IIa HDACs like HDAC4 HDAC5 and HDAC7.
Biological function summary
HDAC9 plays a significant role in regulating gene expression by altering the acetylation status of histones. It participates in the formation of large multiprotein complexes that target specific gene promoters. HDAC9's activity impacts various cellular processes including cell cycle progression and differentiation. It is a part of the CoREST complex which interacts with REST corepressor 1 to modulate neuronal gene expression and synaptic plasticity.
Pathways
HDAC9 is actively involved in key cellular signaling cascades. It contributes to the Notch signaling pathway which has a profound impact on cell differentiation and fate decisions. It also plays a role in the NF-kB pathway which is critical for immune response and inflammation. Through these pathways HDAC9 interacts with proteins such as RBP-Jk in the Notch pathway and IKK complex members in the NF-kB pathway indicating its broad influence on cellular mechanisms.
HDAC9 associates with several pathological conditions. It has been linked to cardiovascular diseases such as cardiac hypertrophy and heart failure due to its influence on cardiac muscle gene expression. HDAC9 also relates to various cancers including colon cancer where its dysregulation affects tumorigenesis and cell proliferation. In these contexts HDAC9 interacts with proteins like MEF2 and p300 which are pivotal in cardiac growth and cancer progression respectively.