Abcam, ab80612, Anti-PH3 antibody

Size: 100µg
Rabbit Polyclonal PH3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Drosophila melanogaster samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human PHC3 aa 700-750.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Drosophila melanogaster,
Applications:WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PHC3 aa 700-750. The exact immunogen used to generate this antibody is proprietary information.Q8NDX5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified using an epitope specific to PH3 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PH3 also known as phospho-Histone H3 (Ser10) plays an important role in cellular division processes. This protein is characterized by its molecular weight and is highly expressed during mitosis particularly in the chromatin of dividing cells. Researchers often refer to PH3 in scientific literature by its molecular mass or specific markers associated with it. The expression of PH3 is typically detected in actively dividing tissues making it a useful tool for identifying proliferation rates in various tissues.
Biological function summary
PH3 is involved in chromatin remodeling during mitosis. It is not part of a larger complex but instead operates as a marker for chromosome condensation. When PH3 undergoes phosphorylation it indicates the cell has entered mitosis. This action facilitates chromosome condensation and segregation critical for proper mitotic progression. Studies often note PH3's significant role during the G2/M transition phase.
Pathways
PH3 operates within vital mitotic and cell cycle regulation pathways. It is closely associated with the CDK1/cyclin B complex which plays an essential part in mitotic initiation. During the cell cycle its phosphorylation state serves as an indicator of mitotic activity. Additionally other proteins such as Aurora B kinase influence PH3's activity further integrating it into the regulatory network controlling cell division.
Increased PH3 expression correlates with various cancers such as breast cancer and melanoma. This overexpression often results from dysregulation of normal cell cycle mechanisms where PH3's activity becomes abnormally high. Oncological studies link PH3 with other proteins such as p53 where mutations can influence the transition from normal cell activity to oncogenesis impacting disease progression and prognosis.