Abcam, ab80680, Anti-MAdCAM1 antibody [AP-MAB0842]

Size: 500µg
Rat Monoclonal MAdCAM1 antibody. Carrier free. Suitable for IP, Flow Cyt, IHC-P, FuncS (Neut/Block), IHC-Fr and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Madcam1 protein.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:AP-MAB0842,
Isotype:IgG2,
Carrier free:Yes,
Reacts with:Mouse,
Applications:FuncS (Neut/Block), Flow Cyt, IP, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing Madcam1 protein. The exact immunogen used to generate this antibody is proprietary information.Q61826

Product details:
Endotoxin Level: Extremely low level of LPS (< 0.002EU/μg IgG).
ab80680 was produced by
in vitro
culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-The IgG fraction of culture supernatant was purified by Protein Aaffinity chromatography., Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAdCAM-1 also known as mucosal addressin cell adhesion molecule 1 or mucosal tag is a protein with a molecular mass of approximately 60 kDa. The MAdCAM-1 protein is expressed prominently on high endothelial venules within mucosal tissues such as the gut and the colon. Additionally it also appears in the endothelial cells of lymphoid tissues. Another name for it is MECA-79 highlighting its recognition by the MECA-79 antibody. Mucosal tags label regions where MAdCAM-1 assists in cell adhesion processes.
Biological function summary
MAdCAM-1 facilitates lymphocyte homing to mucosal tissues. It plays a part in the adhesive interactions between endothelial cells and lymphocytes as a component of the immune surveillance system enabling T cells and B cells to move into areas where they are needed. MAdCAM-1 forms complexes with integrins notably alpha4beta7 which are integral to its adhesion functions. Through its role in guiding lymphocytes it influences immune response and inflammation in mucosal environments.
Pathways
MAdCAM-1 contributes importantly to the trafficking of immune cells. It is involved in the regulation of cell adhesion and migration pathways. The protein interacts extensively with the integrin family especially integrin alpha4beta7 which facilitates lymphocyte entry into the intestinal wall. Additionally it works with chemokine receptors and cytokines that further influence cell movement and homing to specific tissues. This integrative role reinforces its importance in immune response and mucosal immunity.
MAdCAM-1 links closely to inflammatory diseases such as Crohn's disease and ulcerative colitis. Its expression is upregulated in these inflammatory conditions which leads to increased lymphocyte accumulation in the gut. This condition exacerbates the inflammation and tissue damage characteristic of these disorders. MECA-79 a monoclonal antibody targets MAdCAM-1 to aid therapeutic approaches in mitigating inflammation driven by lymphocyte infiltration. Understanding MAdCAM-1 and its function offers potential pathways to target and treat these chronic inflammatory diseases.