Abcam, ab8118, Anti-pro Caspase-12 antibody

Size: 100µg
Rabbit Polyclonal Caspase-12 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Casp12 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Casp12 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.O08736,
Specificity:The antibody only recognizes the prodomain, not the activated form.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pro Caspase-12 also known as ICErel-II or Casp12 is a precursor form of the cysteine-aspartic acid protease Caspase-12. It has a molecular mass of around 48 kDa and is expressed in various tissues with a notable expression in the endoplasmic reticulum (ER). As a precursor protein pro Caspase-12 undergoes cleavage to form the active enzyme which involves apoptotic processes through the proteolytic cascade. This protein plays a mechanical role in the initiation and execution of apoptosis particularly in response to ER stress.
Biological function summary
Pro Caspase-12 involves itself in cellular stress responses especially ER stress-induced apoptosis. It integrates into complexes responsible for mediating cell death signals when ER function is compromised. Known for its function through interactions with other caspases pro Caspase-12 activates effector caspases like Caspase-3 and Caspase-7 which eventually lead to apoptotic cell death. The protein contributes significantly to maintaining homeostasis in response to perturbations within the ER environment.
Pathways
Pro Caspase-12 takes part in the unfolded protein response and ER stress pathways. These pathways are essential for managing misfolded proteins within the ER and preventing toxic aggregation. Through its activity in these pathways pro Caspase-12 connects with proteins such as CHOP a transcription factor that amplifies the apoptotic response under stress conditions and Caspase-9 which is involved in the intrinsic pathway of apoptosis. These interactions highlight its role in coordinating apoptosis triggered by severe ER stress.
Pro Caspase-12 relates to conditions like Alzheimer's disease and type 2 diabetes where ER stress plays a significant role. In Alzheimer's disease the dysregulation of ER stress responses involving pro Caspase-12 associates with neuronal cell death contributing to disease progression. Moreover in type 2 diabetes chronic ER stress links pro Caspase-12 with insulin resistance and pancreatic beta-cell dysfunction. These associations involving proteins like IRE1 and PERK illustrate the importance of pro Caspase-12 in the pathology of ER stress-related diseases.