Abcam, ab81283, Anti-AKT1 (phospho S473) antibody [EP2109Y]

Size: 20µL / 100µL / 1mL
Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) is a rabbit monoclonal antibody detecting AKT1 in Western Blot, IHC-P, ELISA, Dot Blot . Suitable for Human, Mouse, Rat, . - Biophysical QC for unrivalled batch-batch consistency - Over 610 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2109Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, Dot, In-Cell ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:AKT1 (phospho S473) antibody detects AKT1 phosphorylated at Serine 473. The region surrounding S473 in AKT1 is highly similar to the corresponding regions in AKT2 and AKT3, and the immunogen used for this product shares 100% homology with these isoforms. Therefore, cross-reactivity with AKT2 and AKT3 phosphorylated at the equivalent serine residue is possible, although this has not been confirmed experimentally.

Product details:
Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, IHC-P, In-Cell ELISA, WB in human, mouse, rat, recombinant fragment samples.
Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) has been cited over 612 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-phospho AKT antibody (Anti-RAC antibody; anti-pAKT antibody) [EP2109Y] (ab81283) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) has 10 independent reviews from customers.
Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) specifically detects AKT1 Phospho-S473 (UniProt ID: P31749; Molecular weight: 56kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EP2109Y -
ab215873
Antibody clone EP2109Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 488, Alexa Fluor® 647 (
ab194201
ab313593
ab313670
AKT1 (RAC) also known as protein kinase B (PKB), is a crucial component of the PI3K/AKT signaling pathway, which regulates cell growth, survival, and metabolism. It interacts with key cell cycle proteins like p21, p27, and cyclin D1, and tumor suppressors such as p53. In oncology, AKT1 is a significant target due to its role in promoting tumor growth by enhancing cell proliferation and inhibiting apoptosis.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (
ab205718
) and Goat Anti-Rabbit Alexa Fluor® 488 (
ab150077
). Or search our wide range of secondary antibodies for use with your experiment.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKT1 also known as Protein Kinase B (PKB) is a serine/threonine kinase involved in various cellular processes. AKT1 plays an important role in mediating signals for cell survival growth and metabolism. This protein has a molecular weight of approximately 56 kDa. AKT1 is ubiquitously expressed in many tissues including the brain heart and lungs showing its importance in multiple physiological contexts. Phosphorylation of AKT1 at serine 473 denoted as p-AKT S473 is an important modification that regulates its activity.
Biological function summary
AKT1 regulates a broad spectrum of cellular functions through signaling pathways. It is an important player in the phosphatidylinositol 3-kinase (PI3K) and AKT pathway forming part of a complex involved in promoting cell survival and growth. AKT1 interacts closely with other proteins such as mTOR influencing cellular metabolism and autophagy. The phosphorylation state of AKT1 is critical for its activity with modifications like p-AKT S473 impacting its interaction with cellular substrates.
Pathways
AKT1 participates in the PI3K/AKT/mTOR signaling cascade instrumental in cell proliferation and metabolism. AKT1 integrates signals from insulin and growth factors modulating pathways that control cell growth and glucose uptake. This pathway involves proteins like mTOR and phospho-AKT which coordinate cellular responses to external stimuli. AKT1’s phosphorylation at serine 473 and the involvement of AKT1 E17K a gain-of-function mutation influence these pathways significantly.
AKT1 has associations with cancer and metabolic disorders. Dysregulation of the PI3K/AKT/mTOR pathway involving AKT1 and mTOR often results in oncogenic transformation and uncontrolled cellular proliferation. In cancer such as breast cancer AKT1 mutations including AKT1 E17K are implicated altering cell signals for growth. Additionally AKT1 is connected to metabolic disorders such as Type 2 diabetes where it affects insulin signaling and glucose metabolism highlighting its interaction with metabolic proteins like mTOR and phospho-AKT.