Abcam, ab81299, Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

Size: 20µL / 100µL / 1mL
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) is a rabbit monoclonal antibody detecting gamma H2A.X in Western Blot, IP, IHC-P, ICC/IF, Dot Blot . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 270 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP854(2)Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, IHC-P, ICC/IF, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot Blot, ICC/IF, IHC-P, IP and WB.
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) was first used in a scientific publication in 2008 and has been cited over 272 times in peer reviewed journals. It's performance in Western Blot and immunofluorescence in human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) has 16 independent reviews from customers.
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) specifically detects gamma H2A.X Phospho-S139 (UniProt ID: P16104; Molecular weight: 16kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EP854(2)Y -
ab215967
Antibody clone EP854(2)Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, HRP, Alexa Fluor
594, Alexa Fluor
ab195188
ab195189
ab195190
ab206898
ab206900
Gamma H2A.X (γH2A.X) is a phosphorylated form of the H2A histone family member X (H2AX), which plays a crucial role in the cellular response to DNA damage. When double-strand breaks occur in DNA, H2AX is rapidly phosphorylated at serine 139 to form γH2A.X. This modification serves as a marker for DNA damage and is essential for recruiting DNA repair proteins to the site of damage. Researchers use γH2A.X as a sensitive indicator of DNA double-strand breaks in studies of genotoxic stress, cancer and other conditions involving DNA damage and repair.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
Biological function summary
Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.
Pathways
Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.
Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.