Abcam, ab81460, Anti-Factor Va antibody

Size: 1mg
Sheep Polyclonal Factor V antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human F5.
Key facts
Host species:Sheep,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human F5.P12259

Product details:
Concentration 5-10mg/ml (lot specific).

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography, Purification notes-Salt fractionation followed by ion exchange chromatography on DEAE sepharose., Storage buffer-Constituents: 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor Va also known as activated factor V plays a central role in blood coagulation. It forms a part of the prothrombinase complex which accelerates the conversion of prothrombin to thrombin an important step in the clotting process. Factor Va is a cofactor protein with a molecular mass of approximately 330 kDa. It is expressed primarily in platelets and plasma and its availability is increased during coagulation through activation by thrombin.
Biological function summary
Factor Va acts as an important component of the blood coagulation cascade. It forms a complex with factor Xa on phospholipid surfaces creating the tenase complex which is essential for the efficient conversion of prothrombin to thrombin. This complexation greatly increases the enzymatic efficiency of thrombin generation which is important for clot formation and stabilization. Factor Va's activity is regulated by activated protein C which inactivates factor Va to prevent excessive clotting.
Pathways
Factor Va significantly impacts the intrinsic and extrinsic pathways of blood coagulation. It directly interacts with factor Xa in the prothrombinase complex driving thrombin production. Thrombin in turn activates fibrinogen to fibrin promoting clot stabilization. The intrinsic and extrinsic pathways converge on factor Xa making its interaction with factor Va a shared critical point. This relationship emphasizes how factor Va integrates into broader coagulation processes.
Factor Va's role in the coagulation pathway makes it pivotal for conditions like thrombophilia and factor V Leiden. Anomalies in factor Va can lead to inappropriate clot formation contributing to thrombophilic disorders. The factor V Leiden mutation results from a change in the factor V gene rendering factor Va resistant to inactivation by activated protein C therefore increasing thrombotic risk. Understanding these interactions provides insights into pathologies involving coagulopathy.