Abcam, ab81463, Anti-Nup153 antibody [R4C8]

Size: 200µg
Rat Monoclonal Nup153 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nup153 aa 600-1200.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:R4C8,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Rat Nup153 aa 600-1200. The exact immunogen used to generate this antibody is proprietary information.P49791,
Epitope:Amino acids 610-1191 (Zn finger and FG repeats domain).

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography, Purification notes-This antibody was purified from the serum free cultured medium of the hybridoma under mild conditions by propriety chromatography processes. It is filter sterilized., Storage buffer-pH: 6 - 8.5Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nup153 also known as nucleoporin 153 is a component of the nuclear pore complex (NPC) with a molecular weight of approximately 153 kDa. It functions as a scaffold within the NPC which mediates the bidirectional transport of molecules between the nucleus and the cytoplasm. Nup153 is part of the FG-nucleoporin family characterized by domains rich in phenylalanine and glycine repeats. It is highly expressed in the nuclear envelope of eukaryotic cells playing a vital part in maintaining the permeability of the nuclear envelope.
Biological function summary
Nup153 contributes to the assembly and disassembly of the NPC during cell cycle progression. It acts as a docking site for various nuclear import and export receptors therefore is essential for nucleocytoplasmic transport. Nup153 is an important component in the larger nuclear pore complex which itself is made up of multiple nucleoporins forming a sophisticated structure capable of both passive and active transport processes.
Pathways
Nup153 is intricately involved in the nucleocytoplasmic transport pathway and plays a critical role in RNA processing. It interacts with proteins such as Importin β and Exportin which facilitate the transport of different molecules across the nuclear envelope. This pathway is fundamental for the proper regulation of gene expression and cell cycle control exhibiting the importance of Nup153 in cellular dynamics.
Nup153 has been linked to cancer and neurodegenerative diseases. Alterations in Nup153 expression or function can disrupt cellular homeostasis leading to uncontrolled cell proliferation or impaired neuronal function. In cancer it may show altered interactions with p53 an important tumor suppressor protein affecting its regulatory functions. Understanding the role of Nup153 in such diseases may offer insights into potential therapeutic interventions.