Abcam, ab81614, Anti-Wnt3a antibody [3A6]

Size: 100µL
Mouse Monoclonal WNT3A antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WNT3A.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3A6,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human WNT3A.P56704

Product details:
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.
Biological function summary
Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.
Pathways
Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.
Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.