Abcam, ab81874, Anti-PNPLA3 antibody

Size: 100µg
Rabbit Polyclonal PNPLA3 antibody. Suitable for WB and reacts with Human samples. Cited in 18 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PNPLA3 also known as patatin-like phospholipase domain-containing protein 3 is an enzyme that catalyzes the hydrolysis of ester bonds in glycerophospholipids. This protein has a molecular mass of about 53 kDa and is mainly expressed in liver tissue as well as in adipose tissue. It plays a role in lipid remodeling and triglyceride metabolism. The gene for PNPLA3 is located on chromosome 22 and its expression levels can influence its function in cellular lipid dynamics.
Biological function summary
PNPLA3 impacts lipid metabolism and storage in the liver. It does not function as part of a complex but rather exerts its effects independently. PNPLA3 modifies lipid droplet contents by converting triglyceride substrates. This activity helps regulate lipolysis affecting lipid droplet size and number in liver cells. Its function ensures a balance in hepatic lipid levels which is important for maintaining proper liver function and overall metabolic health.
Pathways
PNPLA3 is integral to lipid metabolic pathways and energy homeostasis. It interacts with key players in triglyceride metabolism such as DGAT2 and PNPLA2. These proteins especially DGAT2 help in the transformation and storage of triglycerides reflecting PNPLA3's role in regulating lipid balance. PNPLA3's interactions and functions in these pathways highlight its part in energy storage and utilization processes.
PNPLA3 genetic variants associate with non-alcoholic fatty liver disease (NAFLD) and liver fibrosis. Its role in these conditions relates to its impact on liver triglyceride content. The I148M variant of PNPLA3 has a notable connection to an increased risk of developing NAFLD. Additionally PNPLA3 interacts with proteins involved in fibrogenesis pathways such as TGF-β which influences the progression of liver disease towards fibrosis. Understanding these interactions helps researchers develop targeted therapies for liver conditions.