Abcam, ab8224, Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Size: 100µg / 250µg
Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) is a mouse monoclonal antibody detecting beta Actin in Western Blot, IHC-P, ICC/IF . Suitable for Drosophila melanogaster, Human, Mouse, Rat, Schizosaccharomyces pombe, Xenopus laevis . - Over 520 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:mAbcam 8224,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Xenopus laevis, Drosophila melanogaster, Schizosaccharomyces pombe,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Recognises a single band at 42kD representing beta Actin. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.

Product details:
Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) has been cited over 526 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) has high sensitivity and specificity.
Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) has 35 independent reviews from customers.
Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 100 µg and 250 µg selling sizes.
Conjugation-ready, carrier free format available for antibody clone mAbcam 8224 -
ab178787
Antibody clone mAbcam 8224 is also available pre-conjugated to a variety of labels for your convenience - HRP (
ab197277
Beta Actin (encoded by the ACTB gene) is a crucial component of the cytoskeleton, playing a vital role in cell motility and gene expression. It is commonly used as a beta actin loading control in various experiments to ensure accurate results. Abnormalities in beta Actin are linked to diseases such as cancer and Baraitser-Winter syndrome. Our ACTB antibody are valuable tools for studying these pathways and understanding the molecular mechanisms involved in these conditions.
This monoclonal antibody to beta actin works well as a protein loading control in Western blot for a broad range of species including Xenopus, Drosophila and S. pombe.
This antibody clone [mAbcam 8224] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
orders@abcam.com
or you can find further information

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.