Abcam, ab8245, Anti-GAPDH antibody [6C5] - Loading Control

Size: 100µg
Anti-GAPDH antibody [6C5] - Loading Control (ab8245) is a mouse monoclonal antibody detecting GAPDH in Western Blot, ICC/IF . Suitable for Human, Mouse, Rat . - Over 5100 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:6C5,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Rabbit GAPDH.P46406,
Specificity:This GAPDH antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast. Preliminary data indicates that the GAPDH antibody- loading control ab8245 recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein.

Product details:
Anti-GAPDH antibody [6C5] - Loading Control (ab8245) is a mouse monoclonal antibody and is validated for use in ICC/IF and WB.
Anti-GAPDH antibody [6C5] - Loading Control (ab8245) was first used in a scientific publication in 2003 and has been cited over 5105 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-GAPDH antibody [6C5] - Loading Control (ab8245) has high sensitivity and specificity.
Anti-GAPDH antibody [6C5] - Loading Control (ab8245) has 100 independent reviews from customers.
GAPDH antibodies are often used as loading controls in Western Blot. Anti-GAPDH antibody [6C5] - Loading Control has been verified in Western Blot samples and detects a band at 36kDa Molecular weight.
Anti-GAPDH antibody [6C5] - Loading Control (ab8245) specifically detects GAPDH (UniProt ID: P46406; Molecular weight: 36kDa) and is sold in 100 µg selling sizes.
One of the top cited antibody in the market for GAPDH with >6500 citations and >70 five star reviews. GAPDH is a key target involved in glycolysis and cell metabolism. It plays a crucial role as a housekeeping gene, particularly in understanding GAPDH expression and its function as a metabolic enzyme. GAPDH is widely analysed in GAPDH activity assays and studies of its role in various cellular processes. Additionally, GAPDH is commonly used as a loading control in Western blot and immunocytochemistry/immunofluorescence (ICC/IF) experiments.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Chromatography on protein A Sepharose, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GAPDH also known as glyceraldehyde 3-phosphate dehydrogenase plays a mechanical role in the glycolytic pathway where it catalyzes the sixth step converting glyceraldehyde 3-phosphate into 13-bisphosphoglycerate. This enzyme has a molecular weight of about 36 kDa. GAPDH is ubiquitously expressed in many tissues and cells making it an extensively studied protein in various biological processes. Due to its consistent expression level researchers often use GAPDH as a loading control in western blot experiments to ensure equal protein loading across samples.
Biological function summary
Glyceraldehyde 3-phosphate dehydrogenase contributes not only to energy production through glycolysis but also has roles beyond metabolism. It connects to cellular functions such as apoptosis and acts as a co-factor in RNA binding. Although it is not typically part of a stable protein complex its involvement in numerous cellular functions highlights its importance in maintaining cellular homeostasis.
Pathways
Glyceraldehyde 3-phosphate dehydrogenase integrates into glycolysis the central metabolic pathway for energy production in cells. Besides glycolysis it links to the regulation of apoptosis working alongside proteins like Bcl-2 which modulate cell survival. These pathways demonstrate the protein's critical role in balancing cell energy requirements and programmed cell death.
Abnormalities in GAPDH expression and function relate to neurodegenerative conditions such as Alzheimer's disease and cancer. In Alzheimer's disease GAPDH interactions with proteins like amyloid-beta and tau proteins exacerbate neuronal damage. When overexpressed or dysfunctional in cancer GAPDH supports rapid cancer cell growth and proliferation by enhancing glycolytic flux a behavior known as the Warburg effect.