Abcam, ab83, Anti-TSG101 antibody [4A10] - BSA and Azide free

Size: 100µL
Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting TSG101 in Western Blot, Flow Cytometry, IHC-P . Suitable for Human, Mouse . - BSA, sodium azide, and glycerol-free for easy conjugation - Over 290 publications - Trusted since 1999
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4A10,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Tumor susceptibility gene 101 protein aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q99816,
Specificity:This antibody recognizes the TSG-101 protein, the product of a recently identified tumor susceptibility gene the inactivation of which in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.

Product details:
What is this antibody validated in?
Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of TSG101?
Anti-TSG101 [4A10] - BSA and Azide free (ab83) specifically detects a band for TSG101 (UniProt: Q99816) at a molecular weight of 43kDa.
Trusted by the scientific community
Anti-TSG101 [4A10] - BSA and Azide free (ab83) was first used in a scientific publication in 1999 and has been cited over 290 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE., Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TSG101 also known as Tumor Susceptibility Gene 101 is a protein involved in intracellular trafficking specifically notable within the process of endosomal sorting. It is a component of the ESCRT-I (Endosomal Sorting Complex Required for Transport-I) complex. TSG101 is approximately 46 kDa and is expressed ubiquitously across various tissues. The mechanistic function of TSG101 involves the recognition and ubiquitination of cargo proteins an important step in the formation of multivesicular bodies.
Biological function summary
TSG101 plays a critical role in the sorting and trafficking of proteins destined for lysosomal degradation or recycling back to the plasma membrane. It is an essential part of the ESCRT machinery which also includes other components like VPS37 and VPS28. TSG101 interacts with specific domains and motifs on target proteins facilitating the recognition and recruitment of these proteins into vesicles. Its involvement in membrane budding and scission makes it important for maintaining cellular homeostasis and protein quality control.
Pathways
TSG101 is integrally involved in the ESCRT pathway and is associated with processes like viral budding and cytokinesis. Within these pathways it collaborates with proteins such as ALIX and the ESCRT-II component VPS25 to mediate vesicle formation and facilitate the trafficking of ubiquitinated cargo. The ESCRT pathway is vital in cellular responses such as membrane repair and is important for the life cycle of certain enveloped viruses which utilize host cell machinery for budding.
Defects or alterations in TSG101 expression have correlations with tumor progression and neurodegenerative diseases. Disruptions in the function of TSG101 can affect cellular processes potentially contributing to conditions like cancer and Alzheimer's disease. For example the aberrant activity of TSG101 may interact with proteins like Hrs and ubiquitin ligases Mdm2 in the context of oncogenic signaling pathways which can drive tumor growth and metastasis. Understanding these interactions is key to uncovering potential therapeutic targets within these diseases.