Abcam, ab83306, Anti-SNRP70/U1-70K antibody

Size: 100µg
Anti-SNRP70/U1-70K antibody (ab83306) is a rabbit polyclonal antibody detecting SNRP70/U1-70K in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 20 publications - Trusted since 2010
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of ab83306 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee.

Product details:
What is this antibody validated in?
Anti-SNRP70/U1-70K antibody (ab83306) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of SNRP70/U1-70K?
Anti-SNRP70/U1-70K (ab83306) specifically detects a band for SNRP70/U1-70K (UniProt: P08621) at a molecular weight of 51kDa.
Trusted by the scientific community
Anti-SNRP70/U1-70K (ab83306) was first used in a scientific publication in 2010 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SNRP70 also known as U1-70K is a protein component of the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) complex. It has a molecular mass of approximately 70 kDa. This protein is mainly expressed in the nucleus of eukaryotic cells. SNRP70 binds to the U1 snRNA facilitating the early stages of pre-mRNA splicing. It stabilizes the binding of the U1 snRNP to the 5' splice site therefore playing a role in recognizing and defining correct splice sites necessary for mRNA maturation.
Biological function summary
This protein is integral to the spliceosome a large multi-component complex responsible for removing introns from pre-mRNA. SNRP70 interacts with other core components of the U1 snRNP such as U1A and U1C proteins to assemble the complex. It ensures the accuracy of splice site recognition and contributes to the dynamic rearrangement of the spliceosome during splicing. These interactions importantly influence the regulation of gene expression by facilitating alternative splicing which allows a single gene to code for multiple proteins.
Pathways
SNRP70 is actively involved in the mRNA processing pathway a critical process in gene expression regulation. Its function directly links to the RNA splicing pathway ensuring the generation of mature mRNA from precursor transcripts. SNRP70's role in the spliceosomal complex places it in association with other spliceosome-related proteins such as SF3B1. This interconnectedness with SF3B1 supports the protein's engagement in ensuring accurate splicing and modulating gene expression through alternative splicing events.
Disruptions in SNRP70 function associate with certain autoimmune conditions and neurodegenerative diseases. For instance autoantibodies against SNRP70 are characteristic in patients with systemic lupus erythematosus (SLE) an autoimmune disorder. Also aberrant splicing events involving SNRP70 contribute to the pathology of certain neurodegenerative diseases such as spinal muscular atrophy. In these conditions the misregulation of splicing factors can exacerbate disease progression linking SNRP70 with other autoantigen proteins like Sm proteins in SLE.