Abcam, ab83463, Thioredoxin Reductase Assay Kit (Colorimetric)

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Thioredoxin Reductase Assay Kit (ab83463) is a specific assay for detecting Thioredoxin Reductase (TrxR) activity. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Other biological fluids, Serum,
Assay type:Enzyme activity,
Assay time:40m,
Assay Platform:Microplate reader

Product details:
Thioredoxin Reductase Assay Kit (ab83463) is a specific assay for detecting Thioredoxin Reductase (TrxR) activity.
In the thioredoxin reductase assay protocol, TrxR catalyzes the reduction of DTNB to TNB
in the presence of NADPH, which generates a strong yellow color (ODmax = 412 nm).
Other enzymes present in crude biological samples such as glutathione reductase and glutathione peroxidase can also reduce DTNB. In order to measure TrxR-only activity, a TrxR specific inhibitor is used in a separate reaction to determine TrxR specific activity. The difference between total DTNB reduction in the sample and DTNM reduction in the sample in presence of TrxR inhibitor is the value of specific TrxR activity in the sample.
We have tested the GR positive control from
ab83461
in the conditions of ab83463, and we do not observe activity of the GR positive control.
Thioredoxin reductase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20 min
Other Notes
This product was previously called K763 Biovision Thioredoxin Reductase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Thioredoxin reductase (TrxR, EC 1.8.1.9) is a ubiquitous mammalian enzyme that catalyzes the NADPH-dependent reduction of the redox protein thioredoxin, as well as of other endogenous and exogenous compounds such as selenite, lipid hydroperoxides and hydrogen peroxide.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Thioredoxin reductase also known as TrxR or TXNRD is an enzyme that reduces thioredoxin and other substrates. It is a selenoprotein with a molecular mass of approximately 55 kDa and contains a significant selenocysteine residue at its active site. Thioredoxin reductase exists in cytosol mitochondria and sometimes in the endoplasmic reticulum across various cell types allowing it to fulfill its role in maintaining cellular redox homeostasis by facilitating the reduction of thioredoxin.
Biological function summary
Thioredoxin reductase participates in several critical cellular processes beyond just reducing substrates. It acts as an important player in antioxidant defense cellular signaling and apoptosis. As part of the thioredoxin system which often works closely with other antioxidant systems it plays a role in detoxifying harmful reactive oxygen species. It is not typically part of larger protein complexes but interacts functionally with many proteins to regulate redox signaling pathways.
Pathways
Thioredoxin reductase engages in the regulation of redox signaling and is active in the thioredoxin system pathway. This pathway plays an important role in controlling enzymatic processes that guard cells against oxidative stress. Thioredoxin reductase is also involved in the NADPH-dependent thioredoxin system alongside thioredoxin and NADPH. The enzyme is correlated with the activity of glutathione peroxidase emphasizing its role in redox balance within the cell.
Thioredoxin reductase is implicated in the progression of cancer and cardiovascular diseases. Its overexpression is often observed in cancerous cells where it aids in cell survival and proliferation by managing oxidative stress levels. In cardiovascular diseases altered thioredoxin reductase activity relates to endothelial dysfunction. The enzyme interacts with proteins like thioredoxin to impact disease mechanisms highlighting its importance in therapeutic targets for these conditions.