Abcam, ab8420, Anti-FLASH antibody

Size: 100µg
Rabbit Polyclonal FLASH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Light chain type:unknown,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9UKL3

Product details:
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FLASH also known as "CASP8 and FADD-like apoptosis regulator" is an important protein that plays a role in apoptosis. This protein has a molecular mass of approximately 220 kDa and can be found expressed in various tissues including the liver kidney and heart. FLASH localizes to the nucleus where it interacts with several nuclear components and contributes to critical cellular functions. Researchers recognize its significance in regulating apoptotic pathways making it a target for studies involving cell death.
Biological function summary
The CASP8 and FADD-like apoptosis regulator is part of the complex involved in the regulation of apoptosis and cell cycle. FLASH plays its part by interacting with other apoptotic factors ensuring proper cell cycle progression and programmed cell death. It associates with the death effector domain (DED) proteins and has shown involvement in the activation of caspase-8. This regulation is significant for maintaining cellular homeostasis and preventing the development of cancerous cells.
Pathways
Studies show that FLASH integrates into the apoptotic pathway and NF-kB signaling cascade. It engages in critical interactions with proteins such as FADD and caspase-8 which are essential for the initiation and execution of apoptosis. By influencing NF-kB signaling FLASH modulates inflammatory responses and survival signals effectively balancing cell death and survival mechanisms.
Research frequently connects FLASH to cancer and autoimmune diseases. Its dysregulation can lead to aberrant apoptotic signaling contributing to cancer progression or inappropriate cell death as seen in autoimmune conditions. FLASH’s interaction with proteins like caspase-8 makes it an attractive target for developing therapies for diseases where apoptosis is disrupted. Understanding FLASH’s function and interactions provides insight into potential therapeutic strategies.