Abcam, ab85715, Anti-Glucose Transporter GLUT2 antibody [5D1]

Size: 50µg
Mouse Monoclonal Glucose Transporter GLUT2 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Cell preparation containing SLC2A2 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5D1,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing SLC2A2 protein. The exact immunogen used to generate this antibody is proprietary information.P11168,
Specificity:ab85715 recognizes Glucose Transporter GLUT2 expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the Human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Constituents: PBS, 5% Trehalose, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The glucose transporter GLUT2 also referred to as GLUT 2 GLUT-2 or GLUT2 transporter plays an important role in the movement of glucose across cell membranes. Mechanically it acts as a facilitative transporter allowing glucose and other monosaccharides to enter and exit cells. GLUT2 has a molecular weight of about 55 kDa. High expression of GLUT2 occurs in the liver pancreas kidney and intestines where it facilitates bidirectional glucose transport to maintain glucose balance.
Biological function summary
The role of this transporter is important for glucose sensing and regulation. It is not part of any known complex but operates efficiently as a single unit within its membrane environment. In the pancreas GLUT2 detects blood glucose levels and initiates insulin release from beta cells. In the liver it enables glucose uptake during periods of high blood glucose and facilitates glucose production when blood glucose levels drop. By performing these functions GLUT2 helps maintain glucose homeostasis in the body.
Pathways
GLUT2 is an essential component of glucose homeostasis and nutrient sensing pathways. It links to the insulin signaling pathway by facilitating insulin secretion in response to increased blood glucose. Furthermore GLUT2 associates with kinases such as AMPK which modulate cellular energy balance. The synergistic activity between GLUT2 and insulin signaling pathways highlights its central role in metabolic processes.
Altered GLUT2 function has significant implications. Mutations or deficiencies in GLUT2 can lead to conditions like Fanconi-Bickel syndrome a rare glycogen storage disorder. This transporter has been connected to diabetes as well due to its involvement in insulin secretion regulation. In both these instances GLUT2 interacts with other proteins such as glucokinase in the pancreas and liver highlighting its importance in disease pathology.