Abcam, ab85987, Anti-EpCAM antibody [323/A3]

Size: 100µg
Anti-EpCAM antibody [323/A3] (ab85987) is a mouse monoclonal antibody detecting EpCAM in Western Blot, Flow Cytometry, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Trusted since 2009
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:323/A3,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.P16422,
Specificity:We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Product details:
What is this antibody validated in?
Anti-EpCAM antibody [323/A3] (ab85987) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of EpCAM?
Anti-EpCAM [323/A3] (ab85987) specifically detects a band for EpCAM (UniProt: P16422) at a molecular weight of 35kDa.
Trusted by the scientific community
Anti-EpCAM [323/A3] (ab85987) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-EpCAM antibody [323/A3] (ab85987) has been confirmed by Western blot testing in EPCAM Knockout A431 cells.
Other related products
We have a range of other formats of antibody clone [323/A3] also available for your convenience: ab85987, Alexa Fluor® 647 -
ab253261
, Alexa Fluor® 488 -
ab253268
ab272342

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from cell culture supernatant by protein A affinity chromatography, purity > 95% (by SDS-PAGE)., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary
EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.
Pathways
EpCAM participates in regulatory mechanisms involving the Wnt/Β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.
EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects Β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.