Abcam, ab8805, Anti-pan-AKT antibody

Size: 200µL
Rabbit Polyclonal pan-AKT antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 782 publications. Immunogen corresponding to Synthetic Peptide within Human AKT1 aa 450-500 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AKT1 aa 450-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P31749,
Specificity:Pan Anti-AKT Antibody reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

Product details:
Product Specifications
Anti-pan-AKT antibody (ab8805) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-pan-AKT antibody (ab8805) specifically detects pan-AKT (UniProt ID: P31749; Molecular weight: 56kDa) and is sold in 200 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-pan-AKT antibody (ab8805) has high sensitivity and specificity.
Anti-pan-AKT antibody (ab8805) has been cited over 784 times in peer reviewed journals and is trusted by the scientific community.
Anti-pan-AKT antibody (ab8805) has 11 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Purification notes-This product was prepared from monospecific antiserum by a delipidation and defibrination., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target known as pan-AKT or AKT pan is a serine/threonine kinase involved in several cellular processes. It is also known by the names AKT1 AKT2 and AKT3 reflecting the different isoforms of the AKT protein. This enzyme is approximately 56 kDa in molecular weight although this may slightly vary depending on the isoform. AKT is widely expressed in many tissues including muscles fat and the brain. Scientists often detect the protein via AKT western blot reconfirming its detailed molecular weight and AKT protein size in different tissue samples.
Biological function summary
The AKT protein plays a significant role in cellular signaling regulating cell growth survival and metabolism. The AKT protein does not function alone; it interacts with various partners and is part of the larger signaling complexes. AKT activation requires binding to phosphatidylinositol (345)-trisphosphate a process which is dependent on phosphoinositide 3-kinase (PI3K). This activation leads to downstream effects that are vital for proper cellular functions such as nutrient uptake and glucose metabolism.
Pathways
AKT is an important player in the PI3K/AKT/mTOR pathway one of the most important signaling pathways controlling cell growth proliferation and survival. Within this pathway AKT interacts with many proteins including mTOR and PTEN helping to mediate its diverse biological effects. Additionally the AKT signaling pathway has links to the insulin signaling pathway where it influences the activity of several key metabolic regulators.
AKT activity has connections to cancer and diabetes. Abnormal AKT activation is a feature of many cancers as it can lead to uncontrolled cell growth and survival. In cancer AKT often works alongside other proteins like p53 which are important in tumor suppression and cell cycle regulation. In diabetes altered AKT signaling affects insulin sensitivity as seen with proteins such as IRS1 that interact with the insulin pathway. Understanding AKT's role in these diseases continues to be a focus for developing targeted therapies.