Abcam, ab8898, Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade

Size: 100µg
Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP . Suitable for Cow, Human, Mouse . - Over 1730 publications - Trusted since 2002
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Cow, Human,
Applications:ChIP, ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Histone H3 (tri methyl K9) antibody (ab8898) is specific for Histone H3 tri methyl Lysine 9. Shows slight cross-reactivity with tri methyl K27, which shares a similar epitope (please see Western blot image). Does not react with mono or di methylated K9. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab176916.

Product details:
Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, IHC-P and WB.
Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) was first used in a scientific publication in 2003 and has been cited over 1737 times in peer reviewed journals. It's performance in ChIP, Western Blot, IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) has high sensitivity and specificity.
Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) has 91 independent reviews from customers.
Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) specifically detects Histone H3 Tri Methyl-K9 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.
Top cited antibody for this target with >2000 citations and >60 five star abreviews. Key target to study histone H3 trimethylation, epigenetic regulation and chromatin structure. This antibody is crucial in cancer research, particularly in understanding H3K9me3's role in gene silencing and heterochromatin formation and is widely used in studies of transcriptional repression and histone modifications involving histone methyltransferases. Key histone marker that has been implicated in both transcriptional activation and silencing.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Histone H3 is a core component of the nucleosome playing an important role in DNA packaging within eukaryotic cells. Often referred to as H3 it has a molecular weight of around 15 kDa. Histone H3 is expressed in the nucleus of nearly all eukaryotic cells where it interacts with DNA and other histone proteins to form chromatin structure. Scientists frequently study histone H3 using techniques like Western blotting and immunohistochemistry utilizing antibodies such as anti-histone or phospho-histone H3 to distinguish post-translational modifications.
Biological function summary
Histone H3 participates in chromatin remodeling and gene transcription regulation. As part of the nucleosome complex it influences DNA accessibility and subsequently gene expression. Through post-translational modifications including methylation and phosphorylation H3 modulates chromatin structure. Common modifications include mono- di- and tri-methylation at various lysine residues like K56 which affect transcriptional activation or repression. These modifications form a part of the histone code a framework for regulating chromatin dynamics and genetic information.
Pathways
Histone H3 modifications are integral to several signaling pathways that modulate gene expression and cellular responses. The histone code interpreted by other proteins such as transcription factors and chromatin remodelers contributes significantly to the regulation of the cell cycle and signal transduction pathways like the MAPK/ERK pathway. H3 interacts closely with regulatory proteins including other histones and transcription machinery to facilitate these complex processes.
Histone H3 modifications are associated with various health conditions including cancer and neurological disorders. Aberrations in histone H3 methylation are linked to oncogenic transformations with disruptions often found in cancer types like glioblastoma and breast cancer. The alterations in H3 also connect to Alzheimer's disease through interactions with other histone modifications and their impact on gene expression patterns critical for neural function. Understanding these modifications helps provide insights into disease mechanisms and potential therapeutic targets.