Abcam, ab89692, Recombinant human TAK1 + TAB1 protein

Size: 5µg / 10µg / 100µg
Recombinant human TAK1 + TAB1 protein is a Human Fragment protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>80% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:WB, SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The Specific activity of ab89692 was determined to be 10 nmol/min/mg.,
Accession:O43318,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

Product details:
ab64311
(Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAK1 also known as MAP3K7 is a protein kinase with a molecular mass of approximately 66 kDa. It forms a critical part of the signaling complex through its association with adapter protein TAB1. TAK1 is expressed across various tissues including the heart brain and kidneys. Mechanically it functions by phosphorylating downstream targets initiating key signaling cascades. TAK1's activation requires interaction with specific co-factors such as TAB1 which helps in modulating the enzyme's activity.
Biological function summary
TAK1 and TAB1 play a essential role in immune response and inflammation regulation. As part of a larger signaling complex they engage in cellular responses to cytokines and environmental stress. TAK1 is important for activating transcription factors like NF-kB which then regulates genes involved in immune and stress responses. This ability allows the cell to properly manage stress conditions and maintain homeostasis. Additionally TAK1 is instrumental in cell survival and differentiation processes.
Pathways
TAK1 participates in the MAPK and NF-kB signaling pathways. It acts as an upstream activator transmitting signals from receptors to downstream effectors within these pathways. TAK1 phosphorylates and therefore activates proteins like IKKs in the NF-kB pathway linking extracellular stimuli to nuclear transcription effects. The MAPK pathway involves other related kinases like JNK and p38 which when activated by TAK1 lead to diverse cellular outcomes such as stress response and apoptosis.
Alterations in TAK1 signaling have connections to inflammatory disorders and cancer. Aberrant activation of the NF-kB pathway through TAK1 is linked to inflammatory conditions such as rheumatoid arthritis. In cancer inappropriate TAK1 activity may promote survival and proliferation of cancer cells. TAK1-related processes involve interactions with proteins like TRAF6 in inflammatory and oncogenic pathways which further emphasize the target's significance in disease mechanisms.