Abcam, ab8982, Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker

Size: 100µg
Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982) is a mouse monoclonal antibody detecting Lamin B1 in Flow Cytometry, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Pig . - KO validated for confirmed specificity - Over 40 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:119D5-F1,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human, Pig,
Applications:IHC-Fr, ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Rat Lmnb1.P70615,
Epitope:C-terminal to residue 231.,
Specificity:Reacts with an epitope located C-terminal of residue 231 in lamin B1. Reacts against lamin B1, does not cross react with lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. ab8982 has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested.High levels of background are often observed when staining mouse tissue with a mouse antibody. We recommend ab16048 as an alternative for mouse staining.

Product details:
What is this antibody validated in?
Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Pig samples.
Trusted by the scientific community
Anti-Lamin B1 [119D5-F1] - Nuclear Envelope Marker (ab8982) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Lamin B1 [119D5-F1] - Nuclear Envelope Marker (ab8982) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982) has been confirmed by Immunocytochemistry testing in Lmnb1 Knockout HAP1 cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.
Biological function summary
Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.
Pathways
Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.
Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.