Abcam, ab89982, Recombinant human JAK3 protein (Active)

Size: 20µg
Recombinant human JAK3 protein (Active) is a Human Fragment protein, in the 811 to 1124 aa range, expressed in Baculovirus, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus,
Tags:His tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: 500 pmol/min/μg. Assay conditions: 40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr) substrate and 20 mM ATP. Reaction was done at 30°C for 45 min.,
Accession:P52333,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Preservative: 1.36% ImidazoleConstituents: 20% Glycerol (glycerin, glycerine), 0.63% Tris HCl, 0.58% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
JAK3 also known as Janus kinase 3 is a protein with a molecular mass of approximately 125 kDa. It belongs to the Janus kinase family and plays an important role in the signal transduction pathways. JAK3 is expressed mainly in hematopoietic cells which include white blood cells and related precursor cells. Its primary function involves mediating signaling by associating with the common gamma chain used by cytokine receptors.
Biological function summary
JAK3 is instrumental in the signaling mechanism of the immune system. It participates in the activation of various interleukins specifically those that signal through the common gamma chain receptor complex. JAK3's role is essential in the proper functioning and development of immune cells such as T cells and natural killer cells. Within this framework JAK3 pairs with cytokine receptors transmitting signals that regulate both proliferation and differentiation of these immune cells.
Pathways
JAK3 integrates into JAK-STAT signaling pathways. This protein works alongside STAT proteins including STAT5 to facilitate the transcription of genes involved in cell survival and proliferation. It also interacts with JAK1 another member of the Janus kinase family to form functional complexes. These interactions are pivotal in maintaining normal immune responses and ensuring that signaling pathways are activated appropriately in response to extracellular cues.
Abnormal JAK3 function can lead to immune-related conditions. Severe combined immunodeficiency (SCID) is one such disorder arising from JAK3 mutations that impair immune cell function. Additionally JAK3 dysregulation links to malignancies such as T-cell acute lymphoblastic leukemia. Relating to these disorders JAK3 affects proteins like IL-7R and gamma chains which are important for cytokine signaling contributing to pathologies when malfunctioning.