Abcam, ab90247, Anti-F4/80 antibody [F4/80] - Macrophage Marker

Size: 100µg
Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) is a rat monoclonal antibody detecting F4/80 in ICC/IF . Suitable for Mouse . - Over 30 publications
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:F4/80,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) is a rat monoclonal antibody and is validated for use in ICC/IF in human, mouse samples.
Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) has been cited over 31 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) has high sensitivity and specificity.
Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) specifically detects F4/80 (UniProt ID: Q14246; Molecular weight: 95kDa) and is sold in 100 µg selling sizes.
Antibody clone F4/80 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (
ab204266
ab204467
F4/80 is a marker for murine macrophages, playing a key role in immune responses and inflammation, particularly in diseases like cancer and chronic inflammatory conditions. F4/80 is a key biomarker in chronic inflammatory conditions.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.
Biological function summary
F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.
Pathways
F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.
F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.