Abcam, ab91492, Anti-GOLPH3/MIDAS antibody

Size: 100µg
Rabbit Polyclonal GOLPH3/MIDAS antibody. Suitable for ICC, WB and reacts with Rat samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human GOLPH3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GOLPH3. The exact immunogen used to generate this antibody is proprietary information.Q9H4A6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GOLPH3 also known as MIDAS (Mitochondrial Dynamics Associated) is a protein with a molecular weight of approximately 34 kDa. It is highly expressed in the Golgi apparatus a critical organelle for processing and sorting cellular proteins. GOLPH3 interacts with other proteins and lipids within the Golgi facilitating vesicle budding which is necessary for protein trafficking and secretion. The structural features of GOLPH3 allow it to bind phosphoinositides which indicates its role as a phosphatidylinositol 4-phosphate effector in the Golgi.
Biological function summary
GOLPH3/MIDAS plays a central role in maintaining Golgi structure and functionality. Its involvement in protein trafficking stems from its interaction with the cytoskeleton and Golgi membranes. GOLPH3 is part of a complex that includes several other proteins enhancing its ability to regulate Golgi shape and size which is essential for efficient cellular protein handling. The complex formation allows GOLPH3 to dynamically influence Golgi positioning within the cell an activity important for many cellular processes.
Pathways
The protein GOLPH3 interacts in the mTOR signaling and glycosylation pathways. The protein is associated with the regulation of mTOR signaling through its interaction with proteins like PIK3CA and AKT1. Additionally its role in glycosylation supports protein maturation and trafficking. These pathways are fundamental in cellular growth proliferation and survival and GOLPH3’s involvement highlights its multifunctional nature in cellular homeostasis.
GOLPH3 has connections to cancer and Alzheimer's disease. Its overexpression is linked to tumor development and progression in some cancers as it influences cell proliferation and survival through the mTOR pathway. In Alzheimer's disease abnormal Golgi structure and function have been noted and the dysregulation of GOLPH3 may contribute to the pathology. The association with proteins like tau and amyloid-beta in Alzheimer's further indicates the potential impact of GOLPH3 misregulation on disease progression.