Abcam, ab92373, Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal NEAS antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3830-17,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cleaved NEAS (Asp1185) also known as cleaved Notch extracellular truncation and NEAS is a significant enzymatic cleavage product of the Notch receptor involved in signal transduction. It has a mass of about 300 kDa. Cleavage occurs when the receptor undergoes proteolytic processing producing a functional subunit. This subunit is present in various tissues particularly active in developmental tissues and the nervous system. This localization suggests an important role in those areas for proper cell signaling and differentiation processes.
Biological function summary
The Notch intracellular domain (NICD) which arises after further cleavage is a part of the core Notch signaling pathway. Cleaved NEAS is part of a complex involving other cleavage events; it acts as an intermediary in the Notch signaling cascade. This role makes it important for cellular communication and regulation determining cell fate decisions and controlling differentiation and proliferation. Studies often identify its presence and function within the nuclear and membrane-associated areas indicating roles both in direct gene expression and membrane signaling domains.
Pathways
Cleaved NEAS interacts heavily with Delta/Serrate/LAG-2 (DSL) Notch ligands and influences the Notch signaling pathway. The processed NICD translocates to the nucleus affecting the transcription of target genes involved in developmental processes and immune system regulation. In addition to cooperating with ligands such as Jagged and Delta-like proteins NEAS has interactions that play a part in modulating important pathways like Wnt signaling revealing its role in coordinating diverse signaling networks.
Cleaved NEAS’s dysregulation links it to various cancer types including leukemia and breast cancer. Overactive Notch signaling often due to abnormal cleaved NEAS activity can drive oncogenesis and influence tumor progression. NEAS interacts with regulatory proteins like P53 which is also implicated in tumor suppression making it a point of interest in therapeutic strategies. Additionally its association with neurodegenerative diseases such as Alzheimer's disease indicates its relevance in both proliferative and degenerative conditions further highlighting its biological importance.