Abcam, ab92388, Anti-Rubicon/Baron antibody

Size: 100µg
Rabbit Polyclonal Rubicon/Baron antibody. Suitable for IP, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human RUBCN aa 900 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RUBCN aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q92622

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rubicon also known as Baron is a protein that functions mechanically to regulate autophagy and endosomal maturation. It has a molecular weight of approximately 70 kDa. You can find Rubicon expressed in various tissues but it is especially abundant in the liver spleen and pancreas. The protein acts by interacting with key autophagic and endocytic machinery including Rab7 and UVRAG which modulate vesicular trafficking processes vital for cellular homeostasis.
Biological function summary
Rubicon plays a role as part of the Beclin1-PI3K complex inhibiting autophagic activity. This protein modulates the fusion of autophagosomes with lysosomes a critical step in degrading cellular components. By preventing excessive autophagy Rubicon helps maintain cellular equilibrium balancing degradation and synthesis processes. Its involvement in endo-lysosomal trafficking is essential for the management of cellular waste and nutrient recycling.
Pathways
Rubicon has an important role in the PI3K/AKT signaling and autophagy pathways. Through its interaction with proteins like Beclin1 and Rab7 Rubicon becomes a significant modulator of autophagic flux influencing cellular growth responses and survival. The protein's regulatory function in these pathways impacts how cells respond to stress and nutrient availability linking metabolic demands with appropriate cellular responses.
Aberrant expression of Rubicon connects to cancer and neurodegenerative disorders including Alzheimer's disease. In cancer Rubicon's modulation of autophagy can influence tumor growth and resistance to apoptosis often working in conjunction with Beclin1 to impact tumor dynamics. In Alzheimer's disease the interaction of Rubicon with proteins involved in autophagy and endosomal pathways like Rab7 is associated with the accumulation of pathogenic proteins highlighting its potential role in disease pathogenesis and progression.