Abcam, ab92547, Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Size: 20µL / 100µL / 1mL
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) is a rabbit monoclonal antibody detecting Vimentin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, mIHC . Suitable for African green monkey, Human, Mouse, Rat . - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 1730 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3776,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:ICC/IF, mIHC, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB and mIHC.
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) was first used in a scientific publication in 1976 and has been cited over 1733 times in peer reviewed journals. It's performance in IHC, Western Blot and immunofluorescence in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) has been confirmed by Western Blot testing in Vimentin knockout A549 cells (
ab288984
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) has 91 independent reviews from customers.
Vimentin antibodies are often used as loading controls in Western Blot. Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker has been verified in Western Blot samples and detects a band at 54kDa Molecular weight.
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) specifically detects Vimentin (UniProt ID: P08670; Molecular weight: 54kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR3776 -
ab193555
Antibody clone EPR3776 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
594, Alexa Fluor
488, HRP, Alexa Fluor
647, Alexa Fluor
568, Alexa Fluor
555, PE, Alexa Fluor
405, APC, Alexa Fluor
ab154207
ab185030
ab194718
ab194719
ab202504
ab203428
ab209446
ab210152
ab314250
ab321763
Vimentin is a protein that supports the structural integrity of cells and is often upregulated in cancer cells, contributing to their aggressive behaviour. It plays a role in cancer progression and metastasis, helping tumors evade the immune system.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Vimentin also known by its scientific name VIM is a type of intermediate filament protein that forms part of the cytoskeleton. It has a molecular mass of approximately 57 kDa. Vimentin is expressed in various cell types including mesenchymal cells fibroblasts and endothelial cells. This protein maintains cell integrity and provides resistance against stress by organizing cellular architecture and anchoring organelles. Researchers often use antibodies such as anti-vimentin or anti-mouse vimentin to study its expression and functions in cell biology.
Biological function summary
The role of vimentin involves providing structural support and participating in processes like cell division and wound healing. Vimentin forms complexes with other cytoskeletal proteins significantly impacting cell shape and mobility. Its involvement in cellular stability becomes paramount during events requiring cellular reorganization such as embryogenesis and inflammation. These functions are frequently observed using labeled antibodies like Alexa Fluor 568 and Alexa Fluor 647 for fluorescent imaging.
Pathways
Vimentin plays a role in the epithelial-to-mesenchymal transition (EMT) and interacts within the TGF-beta signaling pathway. It works alongside proteins like N-cadherin and fibronectin in the EMT pathway contributing to increased cell motility and survival. This involvement positions vimentin as a control point reflecting changes in cell phenotypes under physiological and pathological conditions.
Vimentin has relevance in cancer metastasis and cardiovascular diseases. Its aberrant expression correlates with tumor progression and metastatic potential by affecting cell movement and invasion. Vimentin also associates with proteins like collagen and integrins in the context of cardiovascular pathologies influencing vascular stiffness and remodelling. By targeting vimentin researchers aim to understand intervention strategies that may mitigate these diseases.