Abcam, ab92721, Anti-Myosin Light Chain 2 antibody [EPR3741]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Myosin Light Chain 2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 42 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3741,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.
Biological function summary
Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.
Pathways
Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.
Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.