Abcam, ab93561, FITC Anti-H2-Ea antibody [M5/114.15.2]

Size: 100µg
Rat Monoclonal HA21 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 11 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:M5/114.15.2,
Isotype:IgG2b,
Light chain type:kappa,
Conjugation:FITC,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ab93561 reacts with the mouse MHC Class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes.

Product details:
The M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
H2-Ea also known as the I-A alpha chain is a protein with a molecular weight of approximately 33 kDa. It is a part of the major histocompatibility complex (MHC) class II molecules primarily expressed on the surface of antigen-presenting cells including dendritic cells macrophages and B cells. This protein provides a critical mechanical role in presenting peptide antigens to CD4+ T-helper cells facilitating immune responses.
Biological function summary
H2-Ea functions as a component of the MHC class II heterodimer complex participating in antigen processing and presentation. Upon forming a complex with other MHC class II molecules it binds peptides derived from extracellular proteins. This complex formation initiates immune recognition which is essential for the activation of CD4+ T cells. These cells then enable the immune system to tackle pathogens and coordinate the adaptive immune response efficiently.
Pathways
The immune recognition facilitated by H2-Ea is a pivotal step in the adaptive immune pathway. This protein interacts with other MHC molecules and proteins such as CD3 and CD4 during the antigen presentation process. In the pathway H2-Ea plays an important role in ensuring the adaptive immune system correctly activates and regulates responses against foreign antigens and potential threats to the body.
Alterations or deficiencies in H2-Ea expression can impact conditions such as autoimmune diseases. Aberrant H2-Ea function influences the pathogenesis of autoimmune diseases like Type 1 Diabetes and Rheumatoid Arthritis where immune regulation breaks down. The protein's interaction with autoantigens through MHC class II complexes is associated with disease mechanisms and progression often involving other immune-related proteins like Interleukin-6 (IL-6) and Tumor Necrosis Factor (TNF).