Abcam, ab9482, HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Size: 100µL
Anti-GAPDH antibody [mAbcam 9484] - Loading Control - HRP conjugated (ab9482) is a mouse monoclonal antibody detecting GAPDH in Western Blot . Suitable for Human, Mouse, Rat . - Over 220 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:mAbcam 9484,
Isotype:IgG2b,
Light chain type:kappa,
Conjugation:HRP,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of GAPDH?
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 40.2kDa.
Trusted by the scientific community
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) was first used in a scientific publication in 2004 and has been cited over 220 times in peer-reviewed journals.
Reviewed by scientists
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) has over 15 independent reviews from customers.
Related products
Antibody clone mAbcam 9484 is also available pre-conjugated to a variety of labels for your convenience – Anti-GAPDH HRP [mAbcam 9484] - Loading Control (ab9482).
Other related products
We have a range of other formats of antibody clone [mAbcam 9484] also available for your convenience:
ab9484
, Alexa Fluor® 680 -
ab184095
, Alexa Fluor® 790 -
ab184578
, Alexa Fluor® 647 -
ab196697

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-Preservative: 0.1% Proclin 300 SolutionConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.