Abcam, ab9484, Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Size: 100µg
Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) is a mouse monoclonal antibody detecting GAPDH in Western Blot, IHC-P . Suitable for Chicken, Chinese hamster, Cow, Human, Mouse, Pig, Rat, Xenopus laevis . - Over 870 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:mAbcam 9484,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:In western blot, this product typically gives a lower signal in rat lysates compared to human and mouse lysates.

Product details:
For Western blotting, do not use milk for blocking. Our labs have extensively tested the blocking conditions for this antibody and recommend using 5% BSA for 1 hour. The comparison data is shown in the images section.
This antibody clone [mAbcam 9484] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
orders@abcam.com
or you can find further information
Product Specifications
Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) is a mouse monoclonal antibody and is validated for use in IHC-P, WB in chicken, hamster, cow, human, mouse, pig, rat, xenopus laevis samples.
GAPDH antibodies are often used as loading controls in Western Blot. Anti-GAPDH antibody [mAbcam 9484] - Loading Control has been verified in Western Blot samples and detects a band at 36kDa Molecular weight.
Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) specifically detects GAPDH (UniProt ID: P04406; Molecular weight: 36kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) has high sensitivity and specificity.
Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) has been cited over 875 times in peer reviewed journals and is trusted by the scientific community.
Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) has 55 independent reviews from customers.
Related Products
Antibody clone mAbcam 9484 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 680, Alexa Fluor® 790, Alexa Fluor® 647 (
ab9482
ab184095
ab184578
ab196697

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.