Abcam, ab95927, Recombinant human NEDD4 protein

Size: 20µg
Recombinant human NEDD4 protein is a Human Fragment protein, in the 150 to 900 aa range, expressed in Baculovirus, with >60%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>60% SDS-PAGE,
Expression system:Baculovirus,
Tags:DDDDK tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: 0.0027 pmol/min/μg. Assay conditions: 50 μl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotin-Ubiquitin (0.5 μg) and NEDD4 (0-2 μg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against NEDD4, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm). Specific Activity: 0.0027 pmol/min/μg. Assay conditions: 50 μl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotin-Ubiquitin (0.5 μg) and NEDD4 (0-2 μg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against NEDD4, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).,
Accession:P46934,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NEDD4 protein also called NEDD4-1 is an E3 ubiquitin-protein ligase. It has a role in tagging proteins with ubiquitin molecules marking them for degradation by the proteasome. This protein has a molecular mass of approximately 120 kDa. High expression of NEDD4 occurs in tissues like the brain heart and skeletal muscle. The activity of NEDD4 impacts various processes ensuring protein quality control and regulation within cells.
Biological function summary
The impact of NEDD4 protein extends beyond its basic mechanical function. It plays an important role in cell signaling development and even adjusting cell volume. NEDD4 participates in forming a ubiquitin-protein ligase complex engaging with multiple substrates to transfer ubiquitin. It modulates key signaling pathways influencing cellular responses and maintaining healthy cell functioning.
Pathways
NEDD4 activity is embedded in key cellular processes. It is especially integral in the PI3K/AKT signaling and Wnt signaling pathways where it can influence signal transduction and cellular growth. NEDD4 ubiquitin ligase family members like NEDD4-2 are often seen working alongside it. These pathways involve other proteins such as PTEN which NEDD4 regulates affecting cell proliferation and survival.
NEDD4 holds significant connections to cancer and neurodegenerative diseases. Elevated levels of NEDD4 can contribute to oncogenesis particularly in cancers where dysregulation in protein degradation occurs. The aberrant activity of NEDD4 links to neurodegenerative diseases as it affects the turnover of neuronal proteins. It shares a complex relationship with PTEN in diseases considering their interaction can alter cellular pathways often distorted in pathological conditions.