Abcam, ab97523, Anti-SAE1 antibody

Size: 50µL
Rabbit Polyclonal SAE1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAE1 aa 1-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SAE1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q9UBE0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein SAE1 also known as SUMO-activating enzyme subunit 1 functions mechanically as a part of the SUMOylation process. It has a mass of approximately 39 kDa and is expressed in multiple cell types but is mainly found in the nucleus. SAE1 forms a heterodimeric structure with the protein SAE2 and together they are responsible for the initial step of SUMO activation which is important for SUMOylation a post-translational modification process.
Biological function summary
SAE1 is integral to numerous cellular processes that require precise regulation such as transcription DNA repair and signal transduction. It functions as part of the SUMO E1 enzyme complex which is essential for conjugating SUMO proteins to target substrates. This modification influences protein stability localization and interaction adding a layer of control in these cellular activities. The modulation of protein function by SAE1 impacts cell cycle control and stress responses indicating its breadth of influence across cellular mechanisms.
Pathways
The actions of SAE1 significantly impact the PI3K-Akt and DNA damage repair pathways. Its involvement in these pathways highlights its role in regulating cell survival and maintaining genomic integrity. SAE1's interaction within the SUMOylation machinery connects it to other proteins such as Ubc9 the E2 conjugating enzyme which highlights its importance in facilitating modifications necessary for a wide array of cellular functions and signaling cascades.
Defects or dysregulation of SAE1 have been linked to cancer and neurodegenerative conditions. Altered SUMOylation levels controlled by SAE1 can influence tumorigenesis through abnormal cell proliferation or impaired DNA damage responses connecting it with proteins like p53 and PARP1 which are critical in those pathways. Additionally SAE1's involvement in neurological disorders is associated with disrupted protein homeostasis linking it to pathologies where proper cellular regulatory mechanisms are compromised.