Abcam, ab97661, Anti-POLG antibody

Size: 50µL
Rabbit Polyclonal POLG antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLG aa 750 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:IP, ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human POLG aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P54098

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
POLG also known as DNA polymerase gamma is an enzyme essential for mitochondrial DNA replication and repair. It is composed of a catalytic subunit with approximately 140 kDa mass. This protein is mainly expressed in tissues with high energy demands such as liver heart and skeletal muscles. POLG functions by synthesizing the leading and lagging strands during mitochondrial DNA replication ensuring mitochondrial DNA integrity.
Biological function summary
DNA polymerase gamma plays an important role in maintaining mitochondrial genomes. This protein operates within a complex called the POLG complex which is vital for mitochondrial DNA replication machinery. The catalytic subunit works alongside two accessory subunits that enhance its processivity and accuracy during DNA synthesis. Mutations in POLG can impair mitochondrial DNA replication leading to mitochondrial dysfunction.
Pathways
POLG is integral in the mitochondrial DNA replication and repair pathways. It is closely linked with the pathways ensuring mitochondrial gene expression and energy production. POLG acts in concert with mitochondrial DNA helicase TWINKLE and mitochondrial single-stranded DNA binding protein (mtSSB) both contributing to the replication fork activity and stability. POLG's role ensures proper mitochondrial function and consequently cellular energy homeostasis.
POLG mutations have been associated with diverse mitochondrial disorders including Alpers-Huttenlocher syndrome and progressive external ophthalmoplegia. POLG-related disorders often result from impaired mitochondrial DNA replication leading to energy deficits in cells. In particular mutations affecting the POLG complex's performance can influence the onset of these conditions sometimes involving interactions with other proteins like TWINKLE and mtSSB which further complicate the mitochondrial dysfunction seen in such diseases.