Abcam, ab97953, Recombinant Human PDXP protein

Size: 50µg / 250µg
Recombinant Human PDXP protein is a Human Full Length protein, in the 1 to 296 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q96GD0,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein pyridoxal phosphate phosphatase also known as PDXP serves as an important enzyme in the regulation of vitamin B6 bioavailability. It carries a mass of approximately 32 kDa. PDXP dephosphorylates pyridoxal 5'-phosphate (PLP) which is the active form of vitamin B6 to pyridoxal (PL). Primarily the expression of PDXP occurs in the cytoplasm and has been detected in various tissues such as brain and liver suggesting its diverse role across different biological systems.
Biological function summary
Pyridoxal phosphate phosphatase affects many cellular processes by maintaining the balance of pyridoxal 5'-phosphate levels. This enzyme is essential for the regulation of processes that depend on pyridoxal phosphate as a cofactor including amino acid metabolism neurotransmitter synthesis and lipid metabolism. It functions independently rather than being part of a large protein complex allowing its activity to directly influence the availability of pyridoxal phosphate for biochemical reactions.
Pathways
The role of pyridoxal phosphate phosphatase spans significant metabolic pathways such as the amino acid catabolic pathway. Here it functions alongside enzymes that require pyridoxal 5'-phosphate for activity including transaminases and decarboxylases. Additionally PDXP interacts with proteins like pyridoxal kinase which phosphorylates pyridoxal to replenish PLP maintaining the balance between synthesis and degradation in the metabolic flux.
Pyridoxal phosphate phosphatase dysregulation can influence neurological and metabolic disorders. For instance altered PDXP activity has been connected to neurological conditions such as epilepsy where it may affect neurotransmitter levels due to insufficient pyridoxal phosphate. Furthermore mutations in genes related to PDXP or its interacting partners such as pyridoxal kinase could contribute to metabolic syndromes due to disrupted vitamin B6 homeostasis.