Abcam, ab9876, Anti-Mumps nucleoprotein antibody [8H4]

Size: 1mL
Mouse Monoclonal NCAP antibody. Suitable for ICC/IF and reacts with Mumps orthorubulavirus samples. Cited in 8 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:8H4,
Isotype:IgG2b,
Light chain type:unknown,
Carrier free:No,
Reacts with:Mumps orthorubulavirus,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Recognises recombinant yeast-derived mumps NP. Recognises native NP in mumps virus infected Vero cells. This antibody has been tested on 3 different mumps virus strains isolated in the UK: 55799-98, SJ801-95 and Gloucester.

Product details:
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 6.8 - 7.2Preservative: 0.1% Sodium azideConstituents: 15% Fetal Bovine Serum (FBS), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The mumps nucleoprotein sometimes referred to as N protein plays an important role in the replication and transcription of the mumps virus acting as a structural element in the formation of viral ribonucleoprotein (RNP) complexes. The nucleoprotein is a large protein with a molecular mass of approximately 60 kDa and is expressed in cells that are infected by the mumps virus. It facilitates encapsidation of viral RNA and ensures the stability of the viral genome which is essential for virus survival and propagation.
Biological function summary
The mumps nucleoprotein functions as a part of the mumps virus replication complex. It interacts with other viral proteins such as the phosphoprotein (P protein) and the large protein (L protein) to initiate and regulate the replication of viral RNA. These interactions are critical for the stabilization of the entire complex ensuring the efficient production of new viral particles within host cells. The nucleoprotein is primarily located in the cytoplasm of infected cells where it associates with newly synthesized viral RNA strands.
Pathways
The mumps nucleoprotein is involved in the viral replication and transcription pathways specific to the mumps virus. These pathways facilitate viral spread and infection contributing to the virulence of the virus. The nucleoprotein works closely with L and P proteins which serve vital roles in the RNA polymerase complex guiding the synthesis of viral RNA. These interactions are part of the broader negative-strand RNA virus replication pathway shared by other viruses in the Paramyxoviridae family.
The mumps nucleoprotein is directly associated with the mumps disease a contagious viral infection that can lead to severe complications such as encephalitis and orchitis. Antibodies targeting the nucleoprotein can help in diagnosing mumps infections. The nucleoprotein also has implications in mumps-related complications where it interacts with immune system components. Research into this protein helps in understanding the immunological response to the mumps virus potentially informing vaccine development and antiviral therapies.