Abcam, ab98887, Anti-Rhodopsin antibody [Rho 4D2]

Size: 100µg
Anti-Rhodopsin antibody [Rho 4D2] (ab98887) is a mouse monoclonal antibody detecting Rhodopsin in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ELISA . Suitable for Cow, Human, Mouse, Pig, Rat, Xenopus laevis . - Over 50 publications - Trusted since 2010
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Rho 4D2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Cow, Human, Pig, Rat, Xenopus laevis,
Applications:ICC, Flow Cyt, ELISA, WB, IHC-Fr, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Cow Rhodopsin. The exact immunogen used to generate this antibody is proprietary information.P02699

Product details:
What is this antibody validated in?
Anti-Rhodopsin antibody [Rho 4D2] (ab98887) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Cow, Human, Mouse, Pig, Rat, Xenopus laevis samples.
What is the molecular weight of Rhodopsin?
Anti-Rhodopsin [Rho 4D2] (ab98887) specifically detects a band for Rhodopsin (UniProt: P02699) at a molecular weight of 38kDa.
Trusted by the scientific community
Anti-Rhodopsin [Rho 4D2] (ab98887) was first used in a scientific publication in 2010 and has been cited over 50 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rhodopsin also known as visual purple is a protein found in the photoreceptor cells of the retina. It has a mass of approximately 39 kDa. This protein acts as a light-sensitive receptor playing an essential role in the function of rod cells. Rhodopsin is expressed predominantly in the rod outer segment where it transduces light signals. The crystallized form of this receptor referred to as PDB ID: 1D4 has helped researchers study its structure in detail.
Biological function summary
Rhodopsin enables night vision by absorbing photons and activating a G-protein-coupled receptor (GPCR) cascade. Rhodopsin is an integral part of a protein complex within the phototransduction pathway where it works closely with transducin to amplify the visual signal. It initiates the conversion of the photon into an electrical signal. Rhodopsin's role is triggering a structural change in response to light converting from its inactive form to a signaling state.
Pathways
Rhodopsin is deeply involved in the phototransduction and retinoid cycle pathways. Once it absorbs light the transducin it binds causes the activation of a cascade that ultimately results in hyperpolarization of the rod cells. This network of interactions also involves arrestin and recoverin which work together to regulate rhodopsin activity and signal termination. The process ensures precise response and recovery of the visual signal in low-light conditions.
Rhodopsin mutations and malfunctions connect to retinitis pigmentosa and congenital stationary night blindness. Mutations can lead to improper folding or misregulation of the protein impacting its function. Visual cycle proteins like RPE65 are often affected alongside rhodopsin in retinitis pigmentosa. These associations stress rhodopsin's importance in maintaining healthy retinal function and its link to eye diseases.